viernes, 17 de junio de 2016

Efectos bioactivos del extracto de la granada en las líneas celulares sobre la leucemia in vitro

Efectos bioactivos del extracto de la granada en las líneas celulares sobre la leucemia in vitro, una esperanza para los nuevos agentes terapéuticos para la leucemia.

Dahlawi H, JORDAN-Mahy n, clench Mr, el maestro cl.

Fuente

Centro de investigaciones biomédicas, facultad de salud y bienestar, de la universidad de sheffield hallam, Sheffield, Reino Unido.

Abstract

Los estudios indican que la granada  contiene sustancias bioactivas con potencial para el tratamiento y la prevención del cáncer. El extracto de jugo de granada (PJE)  se ha
 demostrado que inhibe la proliferación celular y el crecimiento de tumores e induce la muerte celular por apoptosis en una serie de líneas celulares de cáncer. Sin embargo, hasta la fecha, pocos estudios han investigado el potencial de PJE en el tratamiento de la leucemia. Hemos investigado el efecto potencial de PJE en la inducción de la apoptosis y la inhibición de la proliferación celular en 8 líneas de células de leucemia linfoide (4 y 4) y mieloides nontumor células madre hematopoyéticas (las células de control). La apoptosis fue evaluada por 2 MÉTODOS:....
ARTÍCULO ORIGINAL;
Nutr Cancer. 2012;64(1):100-10. Epub 2011 Nov 18.
Bioactive actions of pomegranate fruit extracts on leukemia cell lines in vitro hold promise for new therapeutic agents for leukemia.
Dahlawi H, Jordan-Mahy N, Clench MR, Le Maitre CL.
Source
Biomedical Research Centre, Faculty of Health and Well Being, Sheffield Hallam University, Sheffield, UK.
Abstract
Studies suggest that pomegranates contain bioactive chemicals with potential for treatment and prevention of cancer. Pomegranate juice extracts (PJE) have been shown to inhibit cellular proliferation and tumor growth and induce cell death via apoptosis in a number of cancer cell lines. However, to date, few studies have investigated the potential of PJE in the treatment of leukemia. We investigated the potential effect of PJE on induction of apoptosis and inhibition of cellular proliferation in 8 leukemia cell lines (4 lymphoid and 4 myeloid) and nontumor hematopoietic stem cells (control cells). Apoptosis was assessed by 2 methods: Annexin V-FITC/propidium iodide staining with flow cytometric analysis and 4'-6-diamidino-2-phenylindole (DAPI) morphological assessment. Cell cycle stage was investigated using propidum iodide staining of DNA content and flow cytometric analysis. Live cell counts were also performed using a trypan exclusion assay. PJE significantly induced apoptosis in all cell lines, including nontumor control cells, although lymphoid cells and 2 of the myeloid cell lines were more sensitive. Furthermore, PJE induced cell cycle arrest. These results were confirmed by DAPI analysis and viable cell counts using trypan blue exclusion assay. Our results provide evidence that PJE contain bioactive compounds that could be used in the treatment of leukemia.

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